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Registros recuperados: 97 | |
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Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan. |
Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms. Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in... |
Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf |
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Arzul, Isabelle; Chollet, Bruno; Michel, Justine; Robert, Maeva; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Miossec, Laurence. |
Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in... |
Tipo: Text |
Palavras-chave: Clam parasites; Perkinsus chesapeaki; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Ribosomal RNA; Actin; ISH. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00094/20542/18205.pdf |
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Thebault, Anne; Martin, Anne-genevieve; Le Coguic, Yvette; Hirata, Toshio; Tige, Gilbert; Pichot, Yves; Chollet, Bruno; Cochennec, Nathalie; Renault, Tristan. |
Le réseau REPAMO répond à plusieurs objectifs, définis dans différentes Directives Européennes: prévenir et détecter l'introduction et la propagation d'un agent pathogène émergent, réémergent ou exotique pour les productions de coquillages d'intérêt commercial en France, surveiller l'évolution des maladies à déclaration obligatoire, surveiller les échanges avec nos partenaires commerciaux. Le fonctionnement repose sur la collaboration entre les professionnels, la DPMCM, les laboratoires côtiers d'IFREMER, et trois cellules d'analyses. Les prélèvements et les outils diagnostiques choisis dépendent des différents objectifs. Le suivi est fait sur des populations sauvages et cultivées du littoral français. Le nombre d'analyses progresse régulièrement grâce à... |
Tipo: Text |
Palavras-chave: Réseau surveillance; Pathologie; Mortalités; Mollusques marins. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00098/20904/18523.pdf |
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Robert, Stephane; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Guilpain, Patrice; Soletchnik, Patrick; Faury, Nicole; Seugnet, Jean-luc; Arnaud, Christophe; Chollet, Bruno; Chabirand, Jean-michel; Fleury, Pierre-gildas; Langlade, Aime; Goulletquer, Philippe; Mille, Dominique. |
Le Bassin ostréicole de Marennes-Oléron, et globalement la Charente-Maritime, sont marqués depuis les années 80 (Héral, 1986), par un net recul des rendements de production qui engendrent une diminution de la rentabilité économique et un accroissement des coûts de production. La démonstration de la surcharge biologique du Bassin de Marennes-Oléron est faite depuis 1985. Cette situation a évolué depuis cette date, par une diminution des cheptels en élevage et par une augmentation des cheptels sauvages ce qui a abouti à des plans d'entretien et de restructuration du DPM. Cependant, la situation défavorable pour la majorité des zones charentaises en terme de croissance-production est annuellement confirmée, depuis 1993 au moyen des réseaux de croissance... |
Tipo: Text |
Palavras-chave: Ostréiculture; Eaux profondes; Crassostrea gigas; Technique élevage; Croissance; Mortalité; Biométrie; Biochimie; Marennes Oléron. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00072/18369/15940.pdf |
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Robert, Stephane; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Guilpain, Patrice; Soletchnik, Patrick; Faury, Nicole; Seugnet, Jean-luc; Arnaud, Christophe; Chollet, Bruno; Chabirand, Jean-michel; Fleury, Pierre-gildas; Bouget, Jean-francois; Goulletquer, Philippe. |
Il est important de rappeler ici les informations concernant le Projet "Culture d'huîtres creuses en eaux profondes dans le Pertuis d'Antioche" (Goulletquer, 2000). Le Bassin de Marennes-Oléron, et globalement la Charente-Maritime, sont marqués depuis les années 80 (Héral, 1985), par un net recul des rendements de production qui engendrent une diminution de la rentabilité économique et un accroissement des coûts de production. La démonstration de la surcharge biologique du Bassin de Marennes-Oléron est démontrée depuis 1985. Cette situation a évolué depuis cette date, par une diminution des cheptels en élevage et par une augmentation des cheptels sauvages ce qui a abouti à des plans d'entretien et de restructuration du DPM. Cependant, la situation... |
Tipo: Text |
Palavras-chave: Ostréiculture; Eaux profondes; Crassostrea gigas; Technique élevage; Croissance; Mortalité; Pertuis Antioche. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/00072/18370/15942.pdf |
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Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Miossec, Laurence; Arzul, Isabelle; Robert, Maeva; Omnes, Emmanuelle; Chollet, Bruno; Renault, Tristan. |
The Ifremer Repamo network (REseau de PAthologie des MOllusques) is the national surveillance network in charge of monitoring marine mollusc health on French coastlines. It is commissioned to enforce the official regulations and to provide a public service, under the supervision of the competent authority (DGAL Veterinary Directorate) in the Ministry.of Agriculture and Fisheries. Its goals are to prevent the introduction and the dissemination of infectious agents, in particular pathogens involved in reportable diseases, and to monitor the changes in the pathogens already present on the French coasts. |
Tipo: Text |
Palavras-chave: Network; Surveillance; Mollusks; Pathogens; Regulations. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6946.pdf |
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Garcia, Celine; Arzul, Isabelle; Chollet, Bruno; Francois, Cyrille; Goubet, Anne; Joly, Jean-pierre; Miossec, Laurence; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Le Gal, Dominique; Rocher, Gregory; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Costantini, Louis; Masson, Jean-claude; Martin, Anne-genevieve. |
Created in 1986, the REPAMO network (Network of mollusc pathology), is a national zoosanitary surveillance network of shellfish health status along the French coasts. Its activities are in keeping with two European Directives, 91 /67/EEC and 95/70/EC and are a patt of the institutional tasks ofIfremer. The aims of the network are to prevent the introduction and spread of exotic pathogens and to survey the evolution of notifiable pathogens already present in France. The network focused on the survey of listed diseases (bonamiosis and marteiliosis of flat oyster) in two areas under agreement process (Banc de Granville and zone X). In 2004, no sample couId be analysed to these two areas because of the difficulty to obtain flat oysters (sparse oyster beds,... |
Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00086/19702/17343.pdf |
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Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle. |
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the... |
Tipo: Text |
Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf |
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Robert, Stephane; Le Moine, Olivier; Razet, Daniel; Guilpain, Patrice; Geairon, Philippe; Arnaud, Christophe; Chollet, Bruno; Faury, Nicole; Soletchnik, Patrick; Seugnet, Jean-luc; Mille, Dominique. |
Le Bassin ostréicole de Marennes-Oléron, et globalement la Charente-Maritime, sont marqués depuis les années 80 (Héral, 1986), par un net recul des rendements de production qui engendre une diminution de la rentabilité économique et un accroissement des coûts de production. La démonstration de la surcharge biologique du Bassin de Marennes-Oléron est démontrée depuis 1985. Cette situation a évolué depuis cette date, par une diminution des cheptels en élevage et par une augmentation des cheptels sauvages ce qui a abouti à des plans d'entretien et de restructuration du DPM. Cependant, la situation défavorable pour la majorité des zones charentaises en terme de « croissance - production» est annuellement confirmée, depuis 1993 au moyen des réseaux de suivi de... |
Tipo: Text |
Palavras-chave: Ostréiculture; Elevage en eaux profondes; Suivis expérimentaux; Biométrie; Mortalité; Bassin de Marennes Oléron; Fouras; Saumonards. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00134/24525/22559.pdf |
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Lupo, Coralie; Osta Amigo, Axel; Fleury, Elodie; Robert, Stephane; Garcia, Celine; Arzul, Isabelle; Baillon, Laury; Bechemin, Christian; Canier, Lydie; Chollet, Bruno; Dechamps, Lucie; Dubreuil, Christine; Faury, Nicole; Francois, Cyrille; Godfrin, Yoann; Lapegue, Sylvie; Morga, Benjamin; Travers, Marie-agnes; Tourbiez, Delphine; Masson, Jean-claude; Verin, Francoise; Cordier, Remy; Gangnery, Aline; Louis, Wilfried; Mary, Charlotte; Normand, Julien; Penot, Julia; Cheve, Julien; Dagault, Francoise; Le Jolivet, Aurore; Le Gal, Dominique; Lebrun, Luc; Bellec, Gwenael; Bouget, Jean-francois; Cochennec-laureau, Nathalie; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Pepin, Jean-francois; Seugnet, Jean-luc; D'Amico, Florence; Maurer, Daniele; Le Gall, Patrik; Mortreux, Serge; Baldi, Yoann; Orsoni, Valerie; Bouchoucha, Marc; Le Roy, Valerian; Pouvreau, Stephane; Queau, Isabelle; Lamoureux, Alice. |
Depuis 1992, la surveillance de la santé des mollusques marins du littoral français est assurée par le réseau de Pathologie des Mollusques (Repamo). Ses activités s’inscrivent dans le cadre de la Directive Européenne 2006/88/CE. Depuis son évaluation par la plateforme nationale d’épidémiosurveillance en santé animale en 2012, l’objectif de surveillance est la détection précoce des infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage. L’année 2015 est la première année de transition pour laquelle un début d’évolution des modalités de surveillance de la santé des mollusques marins animées par l’Ifremer a été amorcé. Un dispositif hybride de surveillance a été mis en place, s’appuyant sur... |
Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Mortalité; Maladie. |
Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00324/43486/42945.pdf |
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Le Roux, Frédérique; Gay, Melanie; Lambert, Christian; Waechter, Magali; Poubalanne, Saravanne; Chollet, Bruno; Nicolas, Jean-louis; Berthe, Franck. |
French mollusc production is based mainly on the Pacific cupped oyster, Crassostrea gigas. Since 1991, annual mass mortality of juveniles has been reported during summer months. These recurring episodes concern professionals who fear that like Portugese oyster, C. angulata, C gigas could in turn disappear following one of these epizooties. Previously, bacteriological analysis of moribund oyster juveniles yielded an isolate of a Vibrio splendidus biovar H strain, named TNEMF6. This isolate was demonstrated to be pathogenic to Crassostrea gigas spat by experimental challenge. To study the association between summer oyster mortality and presence of TNEMF6 cluster strains, Vibrionaceae fauna were isolated from infected spat along the French Atlantic coast... |
Tipo: Text |
Palavras-chave: Vibrio splendidus; Virulence; Epidemiology; PCR RFLP; Oyster mortality. |
Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-565.pdf |
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Francois, Cyrille; Miossec, Laurence; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Robert, Maeva; Ferrand, Sylvie; Chollet, Bruno; Renault, Tristan. |
Created in 1992, the Repamo network (REseau de PAthologie des MOllusques Mollusc Pathology Network) is in charge of the surveillance and monitoring of the marine molluscs health status along the French coast. It has a regulatory mission (European legislation 91/67 and 95/70) as well as a public service activity under the supervision of the Ministry of Agriculture and Fisheries through the DPMA. It aims at preventing the introduction and propagation of pathogenic agents, in particular the noticeable agents, and monitoring the evolution of those already present on the national territory. These activities are part of the institutional missions of the Ifremer. The missions of the network are the following: 1) surveillance of the evolution of noticeable... |
Tipo: Text |
Palavras-chave: Mortalités; Maladies; Pathologie; Mollusques; Réseau surveillance; REPAMO. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-3715.pdf |
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Garcia, Celine; Francois, Cyrille; Arzul, Isabelle; Miossec, Laurence; Joly, Jean-pierre; Robert, Maeva; Chollet, Bruno; Ferrand, Sylvie. |
A monitoring of the natural and high density populations of commercial molluscs is performed on an annual basis within the scope of the Repamo mollusc pathology monitoring network The aim of this surveillance is (1) to monitor the health status of the molluscs along the French coast and to produce a reference picture, and (2) to study the evolution of the noticeable pathogenic agents existing on the national territory. Different sampling strategies have been introduced for the past 15 years; each strategy is based on an active monitoring including the systematic study of natural mollusc deposits. Indeed, the natural and high density mollusc populations are interrelated and pathogenic agents can be and have been exchanged between these populations with... |
Tipo: Text |
Palavras-chave: Haliotis tuberculata; Ostrea edulis; Population sauvage; Elevage; Mollusques; REPAMO; Réseau surveillance. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-3720.pdf |
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Renault, Tristan; Allain, Gwenhael; Arzul, Isabelle; Chollet, Bruno; Cobret, Laetitia; De Decker, Sophie; Faury, Nicole; Ferrand, Sylvie; Francois, Cyrille; Garcia, Celine; Haffner, Philippe; Joly, Jean-pierre; Miossec, Laurence; Morga, Benjamin; Nicolas, Jean-louis; Omnes, Emmanuelle; Pepin, Jean-francois; Saulnier, Denis; Schikorski, David; Segarra, Amelie. |
Abnormal mortality outbreaks occurred in the majority of Pacific oyster (Crassostrea gigas) producing areas in France in 2008 during the summer period. These outbreaks were usually sudden and severe (up to 100%) and affected essentially spat (oysters less than 1 year-old) and juveniles (12 to 18 month-old oysters). Crassostrea gigas was the single affected species Mortality outbreaks were first observed from mid May to mid June in Brittany, Vendée, Charente Maritime and Mediterranean coast A second episode of massive mortality events was reported at the end of June and the beginning of July. All oyster production sites were affected except some particular locations including Arcachon Bay and Brittany. Mortality occurred latter in Arcachon Bay in August.... |
Tipo: Text |
Palavras-chave: Abnormal mortality; Oysters; Crassostrea gigas; Pathogens. |
Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00014/12554/9420.pdf |
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Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the... |
Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf |
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Registros recuperados: 97 | |
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